The ‘Wonder Woman’ actress announced the news on Instagram by sharing a black and white selfie with her husband Yaron Versano.
The couple formed a heart shape with their hands placed on Gadot’s stomach in the sweet snap.
“So excited to share this wonder with you,” she wrote in the caption with the hashtag #mommyforthesecondtime on Sunday 6 November.
The couple, who married in 2008, are already parents to four-year-old Alma.
Gadot, 31, previously opened up about how she finds being a mum.
“I worry that I should always be doing more and spend more time with Alma even when I’m working,” she told HELLO! Magazine in April 2016.
“But I’m very happy being a mother and as soon as you begin raising your child, you feel this incredible love and happiness that is different from anything else you’ve ever experienced.”