The Night Tube is set to run on a section of the London Underground’s Northern line, beginning on the evening of Friday 18 November.
Trains will run overnight on the Charing Cross branch of the Northern line on Friday and Saturday nights.
Transport for London (TfL) said services will be running on average every 8 minutes between Morden and Camden Town and approximately every 15 minutes from Camden Town to High Barnet/Edgware.
There will be no service on the Mill Hill East and Bank branches.
Standard off-peak fares will be charged for travelling on the Night Tube.
This opening brings the total number of Night Tube lines up to four.
The Victoria and Central lines began running overnight services in August, with the Jubilee line joining the night running on 7 October.
The next Night Tube line to open will be the Piccadilly line on 16 December.
Trains will be running on average every 10 minutes between Cockfosters and Heathrow Terminal 5, although there will be no service on the Terminal 4 loop, or between Acton Town and Uxbridge.
Metropolitan, Circle, District, and Hammersmith & City lines are also in the running for Night Tube opening at a later date.