Madonna will perform at half-time during the Super Bowl in February.
NBC, which broadcasts American football's biggest game, announced that the Grammy Award-winning singer will take the stage in Indianapolis on February 5.
The Super Bowl half-time show is the most-watched musical event of the year.
More than 162 million US TV viewers watched The Black Eyed Peas' performance last February.
Madonna, a Rock 'n' Roll Hall of Fame inductee, joins acts including as Bruce Springsteen & The E Street Band, Prince, U2, Paul McCartney, The Who and the Rolling Stones to perform during recent super bowls.
She will collaborate with a team from Cirque du Soleil, choreographer Jamie King and artists from Moment Factory.